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- HOME > °úÇпëÇ° ½ÇÇè±â±¸ ½ÇÇè±âÀÚÀç > ¹°¸®.ÈÇÐ.»ý¹°.¼öÇÐ > Ç¥º».±âŸ > À¯Ã¼ Ƽ¿À±Û¸®ÄÝ ·¹ÀÌÆ®¹èÁö 500gBF-365 [Difco-USA] Fluid thioglycollate Medium(FTM)
Fluid thioglycollate Medium(FTM)
225650 / 500g
Directions for Preparation from
Dehydrated Product
1. Suspend 29.8 g of the powder in 1 L of purified water. Mix thoroughly.
2. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder.
3. Follow label directions specific for each medium.
4. Autoclave at 121¡ÆC for 15 minutes, or as directed on the label.
5. Store fluid thioglycollate media at 15-30¡ÆC. If more than 30% of the medium is pink prior to use,
reheat once (100¡ÆC) to drive off absorbed oxygen.
6. Test samples of the finished product for performance using stable, typical control cultures.
Expected Results
1. Suspend 29.8 g of the powder in 1 L of purified water. Mix thoroughly.
2. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder.
3. Follow label directions specific for each medium.
4. Autoclave at 121¡ÆC for 15 minutes, or as directed on the label.
5. Store fluid thioglycollate media at 15-30¡ÆC. If more than 30% of the medium is pink prior to use,
reheat once (100¡ÆC) to drive off absorbed oxygen.
6. Test samples of the finished product for performance using stable, typical control cultures.
Limitations of the Procedure
Anaerobes can be overgrown by more rapidly growing facultative organisms.
Examine and Gram stain broth if plating medium reveals no growth.
Never rely on broth cultures exclusively for isolation of anaerobes.
Some anaerobes may be inhibited by metabolic products or
acids produced from more rapidly growing facultative anaerobes.
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